Taq polymerase is Pbtained from the bacterium Thermus aquaticus.
The enzyme is thermostable and can withstand the high temperature used for denaturation and separation of the two strands of DNA in PCR. Desired gene can be amplified to produce even a billion copy of DNA.
Thermus aquaticus, a bacterium yields DNA polymerase used in PCR in recombinant DNA technology. (i) This enzyme remains active during the high temperature applied in the denaturation of double stranded DNA. (ii) It extends the primers using the nucleotides provided in the reaction and the genomic DNA as template. (iii) Repeated amplification is achieved by this enzyme. The amplified fragments, if desired can be used to ligate with a vector for further cloning.
Name the source of taq polymerase. Explain the advantage of its use in biotechnology.
Answers
Ravindra
Taq polymerase is Pbtained from the bacterium Thermus aquaticus.
The enzyme is thermostable and can withstand the high temperature used for denaturation and separation of the two strands of DNA in PCR. Desired gene can be amplified to produce even a billion copy of DNA.
Thermus aquaticus, a bacterium yields DNA polymerase used in PCR in recombinant DNA technology.
(i) This enzyme remains active during the high temperature applied in the denaturation of double stranded DNA.
(ii) It extends the primers using the nucleotides provided in the reaction and the genomic DNA as template.
(iii) Repeated amplification is achieved by this enzyme. The amplified fragments, if desired can be used to ligate with a vector for further cloning.