(i) A-Denaturation of the double stranded DNA. (ii) B-Primers (iii) C-DNA polymerase or Taq polymerase.
Importance in PCR It extends the primers using the nucleotides provided in the reaction medium and the genomic DNA as the template. Taq polymerase is thermostable and withstands the high temperature used in denaturation process. Repeated amplification of DNA in PCR is made possible by using thermostable DNA polymerase, which remain active during high temperature.
Mention the three steps involved in each cycle of Polymerase Chain Reaction (PCR). How is repeated amplification of DNA made possible using PCR?
Answers
MR.B.RAJ
(i) A-Denaturation of the double stranded DNA.
(ii) B-Primers
(iii) C-DNA polymerase or Taq polymerase.
Importance in PCR
It extends the primers using the nucleotides provided in the reaction medium and the genomic DNA as the template. Taq polymerase is thermostable and withstands the high temperature used in denaturation process. Repeated amplification of DNA in PCR is made possible by using thermostable DNA polymerase, which remain active during high temperature.