A single stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridise with its complementary DNA in a clone of cells followed by detection using autoradiography.
The clone having the mutated gene will not appear on photographic film, because probe will not be complementary with mutated gene thus, helpful in detecting the presence of mutated gene in cancer patients.
How is recombinant DNA technology help in detecting the presence of mutant gene in cancer patients?
Answers
Ashok basu
A single stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridise with its complementary DNA in a clone of cells followed by detection using autoradiography.
The clone having the mutated gene will not appear on photographic film, because probe will not be complementary with mutated gene thus, helpful in detecting the presence of mutated gene in cancer patients.