Restriction nucleases function by inspecting the length of DNA sequence, and then binding to specific recognition sequences and cutting the strands at sugar phosphate backbones.
These nucleases are of two types depending on their mode of action. (i) Restriction exonucleases cut sequences at terminal ends of DNA. (ii) Restriction endonucleases cut between the two bases of recognition sequence.
How does a restriction nuclease function? Explain.
Answers
Ms. Ayushi Sehgal
Restriction nucleases function by inspecting the length of DNA sequence, and then binding to specific recognition sequences and cutting the strands at sugar phosphate backbones.
These nucleases are of two types depending on their mode of action.
(i) Restriction exonucleases cut sequences at terminal ends of DNA.
(ii) Restriction endonucleases cut between the two bases of recognition sequence.