Creation of recombinant vectors Vector DNA is cut at a particular restriction site by a restriction enzyme, the same that was used to cut the desired DNA segment. The alien DNA is then linked with the vector DNA using enzyme ligase’ to form the recombinant vector. Since, a restriction enzyme Recognises and cuts the DNA at a particular sequence called recognition site, the same restriction enzyme is used for cutting the DNA segment from both the vector and the other source, sp as to produce same sticky ends in both DNA molecules to facilitate their joining.
How are recombinant vectors created? Why is only one type of restriction endonuclease required for creating one recombinant vector?
Answers
Rajesh
Creation of recombinant vectors Vector DNA is cut at a particular restriction site by a restriction enzyme, the same that was used to cut the desired DNA segment. The alien DNA is then linked with the vector DNA using enzyme ligase’ to form the recombinant vector. Since, a restriction enzyme Recognises and cuts the DNA at a particular sequence called recognition site, the same restriction enzyme is used for cutting the DNA segment from both the vector and the other source, sp as to produce same sticky ends in both DNA molecules to facilitate their joining.