(i) Stanley Cohen and Herbert Boyer constructed the first artificial recombinant DNA (rDNA) molecule. (ii) They isolated the antibiotic-resistance gene by cutting out a piece of DNA from a plasmid with the help of restriction enzyme and linked it to a native plasmid of Salmonella typhimurium with the help of DNAIigase.
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
Answers
Dilip Patel
(i) Stanley Cohen and Herbert Boyer constructed the first artificial recombinant DNA (rDNA) molecule.
(ii) They isolated the antibiotic-resistance gene by cutting out a piece of DNA from a plasmid with the help of restriction enzyme and linked it to a native plasmid of Salmonella typhimurium with the help of DNAIigase.