Gel electrophoresis technique works on the principle of separation of DNA fragment on the basis of electric charge. Since, DNA is negatively charged molecule so, they can be forced to separate out according to their size towards anode under an electric field through a medium or matrix (commonly used is agarose). Shorter molecule moves faster and migrate further than the lohger one.
The products obtained through this technique can be utilised as follows: (i) Construction of recombinant DNA by joining with cloning vectors. (ii) Used in making multiple copies of same DNA by using PCR (Polymerase Chain Reaction).
Explain the basis on which the gel electrophoresis technique works. Write any two ways the products obtained through this technique can be utilised.
Answers
Ashima
Gel electrophoresis technique works on the principle of separation of DNA fragment on the basis of electric charge. Since, DNA is negatively charged molecule so, they can be forced to separate out according to their size towards anode under an electric field through a medium or matrix (commonly used is agarose). Shorter molecule moves faster and migrate further than the lohger one.
The products obtained through this technique can be utilised as follows:
(i) Construction of recombinant DNA by joining with cloning vectors.
(ii) Used in making multiple copies of same DNA by using PCR (Polymerase Chain Reaction).