Restriction endonuclease Eco Rl cuts the DNA strands a little away from the centre of the palindromic sequence, but between the same two bases on the two strands, i.e. G and A. 5?-GAATTC-3? 3?- C T T A A G- 5? (i) Due to this, single stranded portions called sticky ends, overhang at the end of each strand. (ii) Because of the stickiness, they easily form hydrogen bonds with their complementary counterparts.
Explain the action of the restriction endonuclease Eco RI.
Answers
Minal
Restriction endonuclease Eco Rl cuts the DNA strands a little away from the centre of the palindromic sequence, but between the same two bases on the two strands, i.e. G and A.
5?-GAATTC-3?
3?- C T T A A G- 5?
(i) Due to this, single stranded portions called sticky ends, overhang at the end of each strand.
(ii) Because of the stickiness, they easily form hydrogen bonds with their complementary counterparts.