Insulin production by Eli Lilly company (i) DNA sequences corresponding to the two polypeptide, A and B-chains of insulin are synthesised in vitro. (ii) They are introduced into plasmid DNA of E. coli. (iii) This bacterium is cloned under suitable conditions. (iv) The transgene is expressed in the form of polypeptides A and B, secreted into the medium. (v) They are extracted and combined by creating disulphide bridge to form human insulin.
Explain how Eli Lilly, an American company, produced insulin by recombinant DNA technology.
Answers
Anu Varma
Insulin production by Eli Lilly company
(i) DNA sequences corresponding to the two polypeptide, A and B-chains of insulin are synthesised in vitro.
(ii) They are introduced into plasmid DNA of E. coli.
(iii) This bacterium is cloned under suitable conditions.
(iv) The transgene is expressed in the form of polypeptides A and B, secreted into the medium.
(v) They are extracted and combined by creating disulphide bridge to form human insulin.