Totipotency of a cell can be defined as the capacity of a cell/explant to grow into a whole new identical plant.
Requirements to produce somaclones of tomato plants are: (i) Explant It is any part of a plant taken out for growing a new plant in special nutrient medium under sterile/aseptic conditions. (ii) Nutrient medium It must have a carbon source such as sugar, inorganic salts, vitamins, amino acids, growth regulators like auxins, cytokinins, etc. (iii) Suitable conditions of light and temperature. This is a tissue culture method by which a number of genetically similar plants called somaclones can be grown. (iv) The plant let grown are later shifted to nurseries for further development and finally to fields.
Define totipotency of a cell. List the requirements, if the objective is to produce somaclones of a tomato plant on commercial scale.
Answers
G.S. Agrawal
Totipotency of a cell can be defined as the capacity of a cell/explant to grow into a whole new identical plant.
Requirements to produce somaclones of tomato plants are:
(i) Explant It is any part of a plant taken out for growing a new plant in special nutrient medium under sterile/aseptic conditions.
(ii) Nutrient medium It must have a carbon source such as sugar, inorganic salts, vitamins, amino acids, growth regulators like auxins, cytokinins, etc.
(iii) Suitable conditions of light and temperature. This is a tissue culture method by which a number of genetically similar plants called somaclones can be grown.
(iv) The plant let grown are later shifted to nurseries for further development and finally to fields.