Amplification of gene is done using polymerase Chain Reaction (PCR). it is carried out in the following steps: (i) Denaturation The double stranded DNA is denatured by applying high temperature of 95°C for 15 seconds. Each separated strand acts as a template. (ii) Annealing Two sets of primers are added, which anneal to the 3? end of each separated strand. (iii) Extension DNA polymerase extends the primers by adding nucleotides complementary to the template provided in the reaction. Taq polymerase is used in the reaction, which can tolerate heat. All these steps are repeated many times to get several copies of the desired DNA.
Any recombinant DNA with a desired gene is required in billion copoies for commercial use. How is the amplification done? Explain.
Answers
Ratan Kumar
Amplification of gene is done using polymerase Chain Reaction (PCR). it is carried out in the following steps:
(i) Denaturation The double stranded DNA is denatured by applying high temperature of 95°C for 15 seconds. Each separated strand acts as a template.
(ii) Annealing Two sets of primers are added, which anneal to the 3? end of each separated strand.
(iii) Extension DNA polymerase extends the primers by adding nucleotides complementary to the template provided in the reaction. Taq polymerase is used in the reaction, which can tolerate heat. All these steps are repeated many times to get several copies of the desired DNA.