ADA deficiency is caused due to the deletion of gene for adenosine deaminase. Methods to cure ADA deficiency are: (i) 1st method In some cases, it can be cured by bone marrow transplantation and enzyme replacement therapy but it is not fully curative. (ii) 2nd method Lymphocytes from patient’s blood were grown in a culture and functional ADA, cDNA was intr* iuced in these lymphocytes using a retroviral vector. The lymphocytes were then transferred into the patient’s body. Periodic infusion of sucH genetically engineered lymphocytes is done because these cells are mortal. (iii) 3rd method This is a permanent method. Genes isolated from the bone marrow cells producing ADA is introduced into cells at early embryonic stage.
What is ADA deficiency? Describe three methods to cure it.
Answers
Ram
ADA deficiency is caused due to the deletion of gene for adenosine deaminase.
Methods to cure ADA deficiency are:
(i) 1st method In some cases, it can be cured by bone marrow transplantation and enzyme replacement therapy but it is not fully curative.
(ii) 2nd method Lymphocytes from patient’s blood were grown in a culture and functional ADA, cDNA was intr* iuced in these lymphocytes using a retroviral vector. The lymphocytes were then transferred into the patient’s body. Periodic infusion of sucH genetically engineered lymphocytes is done because these cells are mortal.
(iii) 3rd method This is a permanent method. Genes isolated from the bone marrow cells producing ADA is introduced into cells at early embryonic stage.