The virus-free healthy sugarcane plant can be obtained through meristem culture of diseased plant; as meristematic tissues are free from viruses they can be used as explants for micropropagation.
Steps in Meristem Culture (i) The shoot tip explants are grown in nutrient medium in either a test tube a pteri dish, under aseptic/sterile in vitro conditions. (ii) The nutrient medium should essentially have a carbon source inorganic salts vitamins, amino acids and growth regulators like auxin and cytokinin in proper defined ratio. (iii) The plantlets, thus obtained are later shifted to nurseries, under observation and finally to fields.
Suggest and describe a technique through which a virus-free healthy plant can be obtained from a diseased sugarcane plant.
Answers
Laxmikant
The virus-free healthy sugarcane plant can be obtained through meristem culture of diseased plant; as meristematic tissues are free from viruses they can be used as explants for micropropagation.
Steps in Meristem Culture
(i) The shoot tip explants are grown in nutrient medium in either a test tube a pteri dish, under aseptic/sterile in vitro conditions.
(ii) The nutrient medium should essentially have a carbon source inorganic salts vitamins, amino acids and growth regulators like auxin and cytokinin in proper defined ratio.
(iii) The plantlets, thus obtained are later shifted to nurseries, under observation and finally to fields.