DNA fragments formed by the use of restriction endonucleases are separated by gel electrophoresis. (i) DNA fragments are negatively charged molecules. Thus, they move towards the anode under electric field through the medium. (ii) DNA fragments separate according to their size due to seiving effect of agarose gel. (iii) The separated DNA fragments can be viewed by staining the DNA with ethidium bromide followed by exposure to UV radiation. (iv) The separated bands of DNA are cut and extracted from gel piece. This is known as elution.
Name and explain the techniques used in the separation and isolation of DNA fragments to be used in recombinant DNA technology.
Answers
Atin Bhardwaj
DNA fragments formed by the use of restriction endonucleases are separated by gel electrophoresis.
(i) DNA fragments are negatively charged molecules. Thus, they move towards the anode under electric field through the medium.
(ii) DNA fragments separate according to their size due to seiving effect of agarose gel.
(iii) The separated DNA fragments can be viewed by staining the DNA with ethidium bromide followed by exposure to UV radiation.
(iv) The separated bands of DNA are cut and extracted from gel piece. This is known as elution.