Thermus aquaticus, a bacterium yields DNA polymerase used in PCR in recombinant DNA technology. (i) This enzyme remains active during the high temperature applied in the denaturation of double stranded DNA. (ii) It extends the primers using the nucleotides provided in the reaction and the genomic DNA as template. (iii) Repeated amplification is achieved by this enzyme. The amplified fragments, if desired can be used to ligate with a vector for further cloning.
How is the bacterium Thermus aquaticus employed in recombinant DNA technology?
Answers
Murti
Thermus aquaticus, a bacterium yields DNA polymerase used in PCR in recombinant DNA technology.
(i) This enzyme remains active during the high temperature applied in the denaturation of double stranded DNA.
(ii) It extends the primers using the nucleotides provided in the reaction and the genomic DNA as template.
(iii) Repeated amplification is achieved by this enzyme. The amplified fragments, if desired can be used to ligate with a vector for further cloning.